ox2 receptor ox2r antagonist  (MedChemExpress)

Journal: Translational Psychiatry

Article Title: Lateral hypothalamus orexinergic projection to the medial prefrontal cortex modulates chronic stress-induced anhedonia but not anxiety and despair

doi: 10.1038/s41398-024-02860-9

Figure Lengend Snippet: A The experimental protocol for the activity of mPFC after RS. B Representative c-Fos positive cells in the mPFC for mice in the Ctrl and RS groups. C The density of c-Fos positive cells in the mPFC region ( n = 4). D Representative photomicrographs of EYFP expression overlapped with neurons expressing orexin-A. E Percentage of EYFP neurons expressing orexin-A, Glu, and GABA ( n = 4). F Top: schematic viral injection plan; Bottom: representative virus expression image in the mPFC. G Left: Representative photomicrographs of mCherry expression overlapped with neurons expressing OX1R and Glu. Right: Percentages of Glu neurons receiving the orexinergic neuron projection in mPFC. H Left: Representative photomicrographs of mCherry expression overlapped with neurons expressing OX1R and GABA. Right: Percentages of GABA neurons receiving the orexinergic neuron projection in mPFC. I Left: Representative photomicrographs of mCherry expression overlapped with neurons expressing OX2R and Glu. Right: Percentages of Glu neurons receiving the orexinergic neuron projection in mPFC. J Left: Representative photomicrographs of mCherry expression overlapped with neurons expressing OX2R and GABA. Right: Percentages of GABA neurons receiving the orexinergic neuron projection in mPFC. K Scheme of experiments. Whole-cell patch-clamp recording on mPFC Glu neurons. L Presentative trace of action potential produced by 250 pA current injection. M A line chart showed the number of action potentials evoked by inward currents in Ctrl and UCMS (Two-way analysis of variance, post hoc least significant difference test, F 1,66 = 17.47, P < 0.0001). N Presentative trace of single action potential induced by the minimal injection current (rheobase). O Scatter plot showed all individual data of the rheobase current ( n Ctrl = 33, n UCMS = 35, from 3 mice, unpaired t test, P = 0.0092). Data are shown as mean ± S.E.M., unpaired t test, * P < 0.05, ** P < 0.01, *** P < 0.001.

Article Snippet: The Orx 1 receptor (Ox1R) antagonist SB334867 (SB, 25 mM, #1455, Tocris Bioscience, Bristol, UK) and Orx 2 receptor (Ox2R) antagonist TCS-OX2-29 (TCS, 33.3 μg/μL, #1457, Tocris Bioscience) dissolved in dimethyl sulfoxide (DMSO, #196055, MP Biomedicals, California, USA) and saline were injected to the mPFC, respectively [ ].

Techniques: Activity Assay, Expressing, Injection, Virus, Patch Clamp, Produced

Journal: Translational Psychiatry

Article Title: Lateral hypothalamus orexinergic projection to the medial prefrontal cortex modulates chronic stress-induced anhedonia but not anxiety and despair

doi: 10.1038/s41398-024-02860-9

Figure Lengend Snippet: A Experimental timeline of microinjection blocking the LH Orx -mPFC pathway. B Left: Schematic of microinjection. Right: Representative image of tube in the mPFC. Blocking either OX1R or OX2R did not prevent anxiety-like ( C time in center of the OFT, D time in open arms of the EPM, E Latency to feed in the NSFT), despair-like phenotypes ( F immobility time in the FST, G Immobility time in the TST). H In the UCMS mice, blocking OX1R or OX2R increased the grooming time in the SST. I Blocking OX1R or OX2R increased volume consumed in the SPT after UCMS. n (Ctrl + DMSO) = 11, n (UCMS + DMSO) = 11, n (UCMS + SB) = 10, n (UCMS + TCS) = 10. Data are shown as mean ± S.E.M., one-way ANOVA, * P < 0.05, ** P < 0.01, *** P < 0.001.

Article Snippet: The Orx 1 receptor (Ox1R) antagonist SB334867 (SB, 25 mM, #1455, Tocris Bioscience, Bristol, UK) and Orx 2 receptor (Ox2R) antagonist TCS-OX2-29 (TCS, 33.3 μg/μL, #1457, Tocris Bioscience) dissolved in dimethyl sulfoxide (DMSO, #196055, MP Biomedicals, California, USA) and saline were injected to the mPFC, respectively [ ].

Techniques: Microinjection, Blocking Assay